In Stage 3 of tissue culture, known as the rooting phase, plantlets are carefully transferred to a specialized rooting medium.
This crucial step is designed to encourage the development of roots, which are essential for the plantlets' transition from the sterile environment of the culture vessel to the outside world.
The rooting medium provides the necessary nutrients and hormonal support to stimulate root growth, helping the plantlets establish a strong root system. As the roots develop, the plantlets are gradually prepared for weaning, where they will be acclimated to their new, less controlled environment.
This phase is vital for ensuring that the plantlets are robust and ready for successful transplantation into soil or larger pots.
Stay tuned for next week: Weaning
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